2024-2-75-81

УДК 615.281.9:615.322
DOI: https://doi.org/10.52540/2074-9457.2024.2.75
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Т. Г. Адамович, Р. И. Лукашов, Д. С. Новаш

ВЫБОР АНТИБАКТЕРИАЛЬНОГО КОМПОНЕНТА ДЛЯ КОМБИНИРОВАННОГО НАЗАЛЬНОГО ПРЕПАРАТА НА РАСТИТЕЛЬНОЙ ОСНОВЕ

Белорусский государственный медицинский университет, г. Минск, Республика Беларусь

В данном исследовании проведена оценка антибактериальной активности извлечения из травы эхинацеи пурпурной, растворов кофейной и хлорогеновой кислот (представителей группы гидроксикоричных кислот), эфирных масел чайного дерева, эвкалипта, лаванды и шалфея и противомикробных лекарственных средств, назначаемых интраназально (сульфацетамида натрия, фрамицетина сульфата, протаргола, неомицина сульфата с полимиксина В сульфатом) в отношении возбудителя бактериального инфекционного ринита Staphylococcus aureus. Оценку бактериостатической активности осуществляли методом последовательного разведения в жидкой питательной среде с использованием индикатора метаболической активности клеток – трифенилтетразолий хлорида, бактерицидной – путем последующего пересева на плотную питательную среду и подсчета колониеобразующих единиц. Сульфацетамид натрия, извлечение из травы эхинацеи пурпурной, кофейная и хлорогеновая кислоты заявленным действием не обладают. Среди изученных эфирных масел наибольшей бактериостатической и бактерицидной активностью обладает эфирное масло чайного дерева и превосходит по данным показателям протаргол. Более сильное действие отмечено для фрамицетина сульфата и неомицина сульфата с полимиксином В сульфатом. По результатам исследования можно рекомендовать эфирные масла чайного дерева, эвкалипта, лаванды и шалфея для включения в состав назальных лекарственных препаратов как более «мягкую» замену антибиотиков.

Ключевые слова: эхинацея пурпурная, кофейная кислота, хлорогеновая кислота, эфирное масло, чайное дерево, лаванда, шалфей, эвкалипт, назальный лекарственный препарат, антистафилококковая активность, бактериостатическое действие, бактерицидное действие.

SUMMARY

G. Adamovich, R. I. Lukashou, D. S. Novash

THE CHOICE OF AN ANTIBACTERIAL COMPONENT FOR A COMBINED HERBAL NASAL PREPARATION

In this study antibacterial activity assessment of extraction from the herb Echinacea purpurea, solutions of caffeic and chlorogenic acids (representatives of hydroxycinnamic acids), tea tree, eucalyptus, lavender and sage essential oils, and antimicrobial preparations administered intranasally (sodium sulfacetamide, framycetin sulfate, protargol, neomycin sulfate with polymyxin B sulfate) in relation to one of the causative agents of bacterial infectious rhinitis Staphylococcus aureus was evaluated. Bacteriostatic activity was evaluated by sequential dilution in liquid nutrient medium using an indicator of the metabolic cells activity - triphenyltetrazolium chloride, and bactericidal activity was evaluated by subsequent re-sowing on dense nutrient medium and counting of colony-forming units. Sodium sulfacetamide, extraction from the herb Echinacea purpurea, caffeic and chlorogenic acids do not have the stated effect. Among the essential oils studied tea tree essential oil has the greatest bacteriostatic and bactericidal activity and exceeds protargol in these indicators. A stronger effect was noted for framycetin sulfate and neomycin sulfate with polymyxin B sulfate. According to the study results tea tree, eucalyptus, lavender and sage essential oils can be recommended for the composition inclusion in nasal preparations as a more “gentle” replacement of antibiotics.

Keywords: Echinacea purpurea, caffeic acid, chlorogenic acid, essential oil, tea tree, lavender, sage, eucalyptus, nasal preparation, antistaphylococcal activity, bacteriostatic effect, bactericidal effect.

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